Zeiss AxioObserver 7- APOllo

The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

Zeiss AxioObserver 7 microscope
Stand modelZeiss AxioObserver 7 APOllo [Inverted]
Microscope controls
  • Fully automated objective nosepiece, reflector turret and condenser
  • Fully motorized stage control (x, y), focus control (z) and light path control
SoftwareZen 3.1 Pro
Capabilities
  • Epifluorescence imaging
  • Brightfield, phase contrast, DIC, Polarised Light (with tunable polariser and analyser)
  • Multi-channel imaging (from 340nm (Fura) to 850 nm (CY7))
  • Timelapse
  • Z-stack
  • Tiling and Multi-position (Unique: 4 slides holder)
  • Physiology module
Objectives
Light sources

Transmitted Light LED

HXP 120 (Metal Halide)

Filters
  • UltraVioletEx: BP 340/30 or BP 387/15 (on Filterwheel), FT: 409 nm, Em: BP 510/90
  • BlueEx: G365, FT: 395 nm, EM: BP 445/50
  • GreenEx: BP 450/50, FT: 480, EM: BP 510/50
  • RedEx: BP 550/25, FT: 570, EM: BP 605/70
  • Far RedEx: BP 640/30, FT: 660, EM: BP 690/50
  • InfraRedEx: BP 710/75, FT: 760, EM: BP 810/90
Camera

Hamamatsu ORCA-Flash LT sCMOS (monochrome) camera

Zeiss Axiocam 705 Color camera

LocationRGN 3144