GE DeltaVision Elite with microinjector

The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

GE DeltaVision Elite with Microinjector microscope
 
Stand modelOlympus XI-71 [inverted]
Microscope controls
  • Fully motorized stage (x, y) and focus control (z)
  • Manual control of nosepieces turret, ocular filters and polychroic filter sets
  • Environmental chamber control (temperature, CO2 and humidity)
SoftwareSoftworx
Capabilities
  • Epifluorescence imaging
  • Brightfield, Phase contrast, DIC
  • Restorative Deconvolution
  • Live cell imaging
  • Multi-channel imaging
  • Timelapse
  • Z-stack
  • Multi-position
  • Tile scanning
  • FRET
Objectives
  • 10x, 0,3NA, Air, UPLFLN (Brightfield, Phase)
  • 20x, 0.75 NA, Air, PLS Apo (Brightfield, Phase)
  • 40x, 1.3 NA, Oil, UPLFLN (DIC, Phase)
  • 60x, 1.4 NA, Oil, Plan-Apo N (DIC, Phase)
  • 100x, 1.3NA, Oil, UPLFLN (Brightfield, Phase)
Light sources
  • Transmitted LED source
  • 7 colour combined Solid State Illumination system (LED)
Filters

3 Polychroic filter combinations

Polychroic 1:

  • Blue Ex 390/18nm, Em 435/18nm
  • Green Ex 475/28nm, Em 525/48nm
  • Red Ex 542/27nm, Em 597/45nm
  • Far Red Ex 632/22nm, Em 679/34nm

Polychroic 2:

  • Blue Ex 390/18nm, Em 435/18nm
  • Green Ex 475/28nm, Em 525/48nm
  • Red Ex 575/25nm, Em 625/45nm
  • Far Red Ex 632/22nm, Em 679/34nm

Polychroic 3:

  • Cyan Ex 438/24nm, Em475/24nm
  • Yellow Em 513/17nm, 548/22nm
  • Red Ex 575/25nm, Em 625/45nm
CameraScientific CMOS Camera
LocationRGN 3140A