Leica Thunder Imager

The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

The Leica Thunder Imager is an upright microscope for fluorescence, transmitted light, and true color imaging. This instrument is equipped with Leica’s computational clearing and deconvolution to remove out-of-focus blur, enhance contrast, and sharpen details. The software Navigator function can be used for rapidly generating sample overviews for subsequent selection of areas of interest.

Instrument specifications

Leica thunder upright microscope
Stand model Leica DM6 B (upright)
Microscope controls
  • Fully automated objective nosepiece, reflector turret and condenser
  • Fully motorized stage control (x, y), focus control (z) and light path control
SoftwareLeica Application Suite X (LAS X) with Leica's thunder algorithms is capable of Instant Computation Clearing (ICC), Small Volume Computation Clearing (SVCC) for cells, yeast, and other thin samples, and Large Volume Computational Clearing (LVCC) for tissue sections, embryos, organoids, and other thick samples.
Capabilities
  • Widefield for acquisition, processing and image analysis.
  • Multi-channel fluorescent imaging (Dapi to CY7)
  • Time series
  • Z-stack
  • Tiling (Leica Navigator)
Objectives
  • 1.25×/0.04 HC PL Fluotar | DRY objective
  • 5×/0.12 N PLAN | DRY objective
  • 10×/0.32 HC PL Fluotar | DRY objective
  • 40×/0.95 HC PL Apo CORR | DRY objective
  • 20×/0.75 HC PL Apo IMM CORR CS2 | Multi-Immersion
  • 63×/1.40-0.6 HC PL Apo Oil | oil Immersion
Fluorescence / Reflected light Source & Filter Sets
  • Fluorescent light source: EL6000
  • Filter name      | excitation  | Dichroic mirror   | Emission
    • DAPI                350/50            400                   460/50
    • GFP                 470/40            495                    525/50
    • Cy3                 545/25            565                    605/70
    • Texas Red       560/40            585                    630/75
    • Cy5                  620/60            660                    700/75
    • Cy7                  710/75             760                    810/90
Transmitted Light & Contrasting Method
  • Transmitted light source: LED
  • Bright Field
Camera
  • Leica K5 sCMOS monochrome camera , 2048 x 2048 pixels (Fluorescence)
  • Leica Flexacam C3 couleur camera , 12 MP CMOS sensor (Histochemistry staining)
Location  RGN 3142