The goal of the CBIA core is to help users getting high quality data efficiently.

Plan your visit

Never hesitate to ask any question to our Microscopy specialist. From sample preparation to acquisition and image analysis we are here to help!

Users are encouraged to discuss experimental design details with CBIA Core staff in order to pick the most suitable system for carrying out planned experiments.

Sound knowledge of research question and fluorophores is preferable for an effective consultation.

To collect proper data from imaging, you need to prepare ahead. Ask yourself:  

  • What is the scientific question you want to answer?
  • Where is your signal located in the cells?
  • What controls are needed to distinguish a meaningful experimental result?
  • What is the resolution you need?
  • How many dimensions? (x, y, z, t, multi-color)
  • Do you need to fix your samples or use live specimen?
  • How many fluorophores do I have to image?
  • If live imaging required, what are you expecting in terms of movements? For how long will you need to image? At what frame rate? Do you need environmental control?
  • While considering which dye or fluorescent probes to use, or how to prepare your samples check out Spectral Database resources in the useful links section of our website.
  • If you are not sure contact us to check the compatibility of your experimental design and needs with the equipment offered by the CBIA Core.

Most objectives in the CBIA core facility are corrected to work with #1.5 coverslips, 0.17mm thick, please check that this is what your lab has in stock before starting any experiments.

Some systems within the CBIA Core have correction collars which allow for an adjustable long working distance, LD objectives, if you require use of these objectives, for example to image through plastic culture dishes please contact CBIA Core staff.

Contact us

Cell Biology and Image Acquisition Core Facility

Faculty of Medicine
University of Ottawa
RGN 3171
451 Smyth Road
Ottawa, ON K1H 8M5

[email protected]

Who to contact?

Core Director
John W.R. Copeland, PhD
Phone: 613-562-5800 ext. 8641

[email protected]

Microscopy specialist Senior and Facility Manager
Chloë van Oostende-Triplet, PhD
Phone: 613-562-5800 ext. 8376

[email protected]

Microscopy specialist
Liyuan Wang, PhD
Phone: 613-562-5800 ext. 8983

[email protected]