BD LSRFortessa – 16-colour Analyzer

The FCV Core Facility currently houses the following instruments: BD LSRFortessa SORP, BD Celesta, Beckman Coulter MoFlo Astrios, and an AutoMACS Pro Magnetic Cell Separator (located at OHRI).

Instrument specifications

Lasers

300mW – 488nm (Blue) solid state
50mW - 561nm (Yellow-Green) solid state
40mW – 640nm (Red) solid state
50mW – 405nm (Violet) solid state

Sample loading options

  • High-Throughput Sampler for 96-well and 384-well plates
  • 12x75mm 5ml polystyrene tubes (Falcon 352052)
Fortessa Configuration: 4 Lasers – 16 colours
Fortessa Configuration: 4 Lasers – 16 colours

Configuration
 

LaserABCDEFGH
405nmN/A780/60: BV786, QDot800710/50: BV711, QDot705660/20: BV650, QDot655N/A610/20: BV605, QDot605530/30: BV510, V500, QDot525450/50: Pacific Blue, BV421,V450
488nm695/40: PerCP-Cy5.5530/30: FITC, eGFP, BB515, AF488SSCN/AN/AN/AN/AN/A
561nm780/60: PE-Cy7710/50: PE-Cy5.5670/30: PE-Cy5610/20: mCherry, PE-CF594586/15: PE, Ds-Red, PIN/AN/AN/A
640nm780/60: APC-Cy7, APC-H7, APC-Fire750730/45: AF700660/20 APC, AF647N/AN/AN/AN/AN/A